ABSTRACT
Objective To study the effect of vibration on free hemoglobin(FHb), lactic dehydrogenase(LDH), and K+concentration of red blood cells(RBCs)stored for different lengths of time.Methods Thirty-five bags of RBCs stored for different lengths of time(3,7,11,26,and 35 d,7 bags per group)were chosen as the target of research.A wheeled vehicle that could vibrate in three directions(horizontal,longitudinal,and vertical)was used to simulate RBC transportation in addition to an electromagnetic vibration test system.The RBC samples were prepared and vibrated for 0 h,or 3 h for detection and analysis of their FHb, LDH, and K+concentration.Results The FHb concentration was significantly increased after vibration(P<0.05), and tended to increase with the extension of storage time.LDH(P<0.05)and K +concentrations(P<0.05)were obviously increased with the length of storage(3,7,11,26,and 35 d), and significantly increased after vibration of 3 h.Conclusion FHb concentration is increased after vibration, and the storage time could change FHb.The vibration and storage time have great effect on LDH and K +concentrations.